The performance of point-of-care antibody test for COVID-19 diagnosis in a tertiary hospital in Bandung, Indonesia

  • Nisa Fauziah Division of Parasitology, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
  • Raspati C Koesoemadinata Infectious Disease Research Center, Faculty of Medicine, Universitas Padjadjaran, Bandung Indonesia
  • Basti Andriyoko Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran – Dr Hasan Sadikin General Hospital, Bandung, Indonesia
  • Lia Faridah Division of Parasitology, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
  • Silvita F Riswari Division of Parasitology, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
  • Leonardus Widyatmoko Indonesian Society of Clinical Microbiology, Bandung, Indonesia
  • Delita Prihatni Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran – Dr Hasan Sadikin General Hospital, Bandung, Indonesia
  • Savira Ekawardhani Division of Parasitology, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
  • Azzania Fibriani School of Life Science and Technology, Institute Teknologi Bandung, Bandung, Indonesia
  • Emma Rachmawati West Java Provincial Reference Laboratory, Bandung, Indonesia
  • Ryan B Ristandi West Java Provincial Reference Laboratory, Bandung, Indonesia
  • Cut NC Alamanda West Java Provincial Reference Laboratory, Bandung, Indonesia
  • Susantina Prodjosoewojo Infectious Disease Research Center, Faculty of Medicine, Universitas Padjadjaran, Bandung Indonesia
  • Bony W Lestari Infectious Disease Research Center, Faculty of Medicine, Universitas Padjadjaran, Bandung Indonesia
  • Ida P Santoso Infectious Disease Research Center, Faculty of Medicine, Universitas Padjadjaran, Bandung Indonesia
Keywords: SARS-CoV-2, IgM/IgG test, point-of-care testing

Abstract

Introduction: We aim to describe the performance of combined IgM and IgG point-of-care antibody test (POC-Ab) (Wondfo®) compared to real-time reverse transcriptase (rRT-PCR) (Allplex™ 2019-nCoV Assay) in detecting coronavirus disease 2019 (COVID-19).

Methodology: We compared POC-Ab with rRT-PCR results among patients in a tertiary hospital from January to March 2020 in Bandung, Indonesia. We selected presumptive COVID-19 patients with positive rRT-PCR consecutively and 20 patients with negative rRT-PCR results were selected randomly from the same group of patients as controls. We described the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) with corresponding 95% confidence interval using serum and capillary blood samples. We also tested POC-Ab using non-COVID-19 (confirmed dengue and typhoid) patients’ sera.

Results: Twenty-seven patients with positive rRT-PCR result and 20 negative controls were included (68.1% males, mean age 46 (SD: 15.4)). Using the serum, the sensitivity of the POC-Ab was 63.0% (42.4-80.6), specificity was 95.0% (75.1-99.9), PPV was 94.4% (72.7-99.8), NPV was 65.5% (45.7-82.1). A subset of 20 patients was tested using a capillary blood sample. The accuracy of the capillary blood sample is lower compared to serum (50.0% vs. 78.7%). None of the non-COVID-19 sera tested were reactive.

Conclusions: POC-Ab for COVID-19 has a high specificity with no false-positive result in non-COVID-19 sera. Therefore, it can be used to guide diagnostic among symptomatic patients in resource limited settings. Given its low sensitivity, patients with high suspicion of COVID-19 but non-reactive result should be prioritized for rRT-PCR testing.

Published
2021-03-07
How to Cite
1.
Fauziah N, Koesoemadinata RC, Andriyoko B, Faridah L, Riswari SF, Widyatmoko L, Prihatni D, Ekawardhani S, Fibriani A, Rachmawati E, Ristandi RB, Alamanda CN, Prodjosoewojo S, Lestari BW, Santoso IP (2021) The performance of point-of-care antibody test for COVID-19 diagnosis in a tertiary hospital in Bandung, Indonesia. J Infect Dev Ctries 15:237-241. doi: 10.3855/jidc.13370
Section
Coronavirus Pandemic