Anti-Trypanosoma cruzi activity, cytotoxicity and, chemical characterization of extracts from seeds of Lonchocarpus cultratus

  • Aline Griebler Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil
  • Fernanda Weyand Banhuk Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil https://orcid.org/0000-0002-3549-8369
  • Izabela Virginia Staffen Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil https://orcid.org/0000-0001-9246-1426
  • Aline Antunes Maciel Bortoluzzi Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil
  • Thaís Soprani Ayala Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil https://orcid.org/0000-0002-2080-2516
  • Rinaldo Ferreira Gandra Laboratory of Microbiology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil https://orcid.org/0000-0001-9586-7253
  • Ivânia Teresinha Albrecht Schuquel Department of Chemistry, Center of Exact Sciences, State University of Maringá, Maringá, PR, Brazil https://orcid.org/0000-0001-6314-4448
  • Edson Antonio Alves da Silva Center of Exact and Technological Sciences, Western Parana State University, Cascavel, PR, Brazil
  • Tereza Cristina Marinho Jorge Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil https://orcid.org/0000-0001-5115-8455
  • Rafael Andrade Menolli Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil https://orcid.org/0000-0003-2728-8318
Keywords: Chagas’ disease, chalcones, trypanocidal activity, cytotoxicity, hemolytic activity

Abstract

Introduction: Trypanosoma cruzi is the agent of Chagas’ disease and affects approximately 6-8 million people worldwide. The search for new anti-T. cruzi drugs are relevant because only two drugs exist actually. The objective of this study was to investigate the effect of the extracts from the seeds of Lonchocarpus cultratus on T. cruzi, its cytotoxicity as well as to elucidate its chemical profile.

Methodology: The characterization of the extracts was done using 1H-RMN. T. cruzi forms were treated with increasing concentrations of the extracts and after, the percentage of inhibition and IC50 or LC50 were calculated. Murine peritoneal macrophages were treated with different concentrations of the extracts to evaluate the cellular viability. The hemotoxicity was accessed by verifying the levels of hemolysis caused by the extracts on human red blood cells.

Results: Chalcones isocordoin and lonchocarpin were detected in the dichloromethane extract, and chalcone lonchocarpin was detected in the hexane extract. The dichloromethane extract showed higher activity against all the forms of T. cruzi compared to the other two extracts, but the hexane showed the best selectivity index. The cytotoxicity observed in murine macrophages was confirmed in human erythrocytes, with dichloromethane extract having the highest toxicity. The methanolic extract showed the lowest anti-T. cruzi activity but was nontoxic to peritoneal murine macrophages and red blood cells.

Conclusions: L. cultratus extracts have the potential to be explored for the development of new anti-trypanosomal drugs. This study was the first to demonstrate the action of extracts from the genus Lonchocarpus on infecting forms of T. cruzi.

Author Biographies

Aline Griebler, Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences

Fernanda Weyand Banhuk, Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences

Izabela Virginia Staffen, Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences

Aline Antunes Maciel Bortoluzzi, Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences

Thaís Soprani Ayala, Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences

Rinaldo Ferreira Gandra, Laboratory of Microbiology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Microbiology, Center of Medical and Pharmaceutical Sciences

Ivânia Teresinha Albrecht Schuquel, Department of Chemistry, Center of Exact Sciences, State University of Maringá, Maringá, PR, Brazil

Department of Chemistry, Center of Exact Sciences

Edson Antonio Alves da Silva, Center of Exact and Technological Sciences, Western Parana State University, Cascavel, PR, Brazil

Center of Exact and Technological Sciences

Tereza Cristina Marinho Jorge, Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Phytochemistry, Center of Medical and Pharmaceutical Sciences

Rafael Andrade Menolli, Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, PR, Brazil

Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences

Published
2021-03-07
How to Cite
1.
Griebler A, Weyand Banhuk F, Staffen IV, Antunes Maciel Bortoluzzi A, Soprani Ayala T, Ferreira Gandra R, Schuquel ITA, Alves da Silva EA, Marinho Jorge TC, Andrade Menolli R (2021) Anti-Trypanosoma cruzi activity, cytotoxicity and, chemical characterization of extracts from seeds of Lonchocarpus cultratus. J Infect Dev Ctries 15:270-279. doi: 10.3855/jidc.12669
Section
Original Articles