Investigation of NDM, VIM, KPC and OXA-48 genes, blue-carba and CIM in carbapenem resistant Enterobacterales isolates

Yeliz Tanriverdi Cayci; Ilknur Biyik; Ferhan Korkmaz; Asuman Birinci

doi:10.3855/jidc.13345

Investigation of NDM, VIM, KPC and OXA-48 genes, blue-carba and CIM in carbapenem resistant Enterobacterales isolates

Authors

Yeliz Tanriverdi Cayci Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey
Ilknur Biyik Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey
Ferhan Korkmaz Rize State Hospital, Microbiology Laboratory, Rize, Turkey
Asuman Birinci Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey

DOI:

https://doi.org/10.3855/jidc.13345

Keywords:

Carbapenem Resistance, Enterobacteriaceae, Blue Carba, CIM

Abstract

Introduction: Carbapenem resistance is an emerging problem in Enterobactarales. We aimed to investigate the presence of carbapenemase genes bla_NDM, bla_KPC, bla_VIM and bla_OXA-48 and evaluate the phenotypic blue-carba method and carbapenem inactivation method (CIM) in Enterobacterales isolates.

Methodology: Total of 153 Enterobacterales isolates were tested in the study. Presence of bla_NDM, bla_KPC, bla_VIM and bla_OXA-48 genes was investigated by polymerase chain reaction (PCR) method. Carbapenemase production of the isolates was also tested by blue-carba method and CIM.

Results: The presence of bla_OXA-48 gene was detected in 110 (71.4%) and bla_NDM gene was detected in 2 (1.3%) of the Enterobacterales isolates by PCR method. None of the isolates were positive for bla_KPC and bla_VIM genes. The 121 (78.54%) of the isolates were found to be positive by blue-carba method and CIM. And 105 (68.18%) of the isolates were determined as positive by both PCR, blue-carba and CIM.

Conclusions: In our study, 112 (72.7%) of the Enterobacterales isolates were found to be positive for carbapenemase genes (bla_oxa-48 and bla_NDM), and 121 (78.57%) of different isolates were found to be positive for blue-carba and CIM. However, 105 (68.18%) of the carbapenem resistance isolates found to be positive for all three methods.

Author Biography

Yeliz Tanriverdi Cayci, Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey

department of Medical Mİcrobiology

Downloads

Published

2021-05-31

How to Cite

Tanriverdi Cayci Y, Biyik I, Korkmaz F, Birinci A (2021) Investigation of NDM, VIM, KPC and OXA-48 genes, blue-carba and CIM in carbapenem resistant Enterobacterales isolates. J Infect Dev Ctries 15:696–703. doi: 10.3855/jidc.13345

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 15 No. 05: May 2021

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).