Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon

  • Silvia Cristina da Silva Pedroso Post-graduate Program in Animal Science, Federal University of Pará, Belém, Brazil
  • Karla Valéria Batista Lima Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Brazil
  • Ismari Perini Furlaneto Post-graduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, Brazil
  • Yan Corrêa Rodrigues Post-graduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, Brazil
  • Darlene Kássia Saraiva Queiroz Pantoja Post-graduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Brazil
  • Alex Junior Souza de Souza Max Planck University Center, Indaiatuba, Brazil
  • Washington Luiz Assunção Pereira Institute of Animal Health and Production, Federal Rural University of Amazonia, Belém, Brazil
Keywords: Granuloma, mycobacteriosis, non-TB mycobacteria, sanitary inspection, zoonosis

Abstract

Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region.

Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively.

Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region.

Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil.

Published
2021-05-31
How to Cite
1.
da Silva Pedroso SC, Batista Lima KV, Perini Furlaneto I, Corrêa Rodrigues Y, Saraiva Queiroz Pantoja DK, Souza de Souza AJ, Assunção Pereira WL (2021) Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon. J Infect Dev Ctries 15:736-741. doi: 10.3855/jidc.13558
Section
Brief Original Articles