Serologic evidence of Echinococcus granulosus in slaughterhouses in Pakistan: global alarm for butchers in developing countries

  • Mughees Aizaz Alvi State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China
  • Li Li State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China
  • Muhammad Saqib Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan
  • John Asekhaen Ohiolei State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China
  • Muhammad Waqar Younas Punjab Livestock and Dairy Development Department, Government of Punjab, Pakistan
  • Muhammad Haleem Tayyab Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan
  • Ali Hassan Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan
  • Warda Qamar Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore (Jhang Campus), Pakistan
  • Anum Aizaz Alvi Independent Researcher, Faisalabad, Punjab, Pakistan
  • Bao-Quan Fu State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China
  • Hong-Bin Yan State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China
  • Wan-Zhong Jia State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory of Animal Hydatidosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, China
Keywords: Butchers, ELISA, Echinococcus granulosus, occupational risk, Pakistan, seroepidemiology

Abstract

Introduction: Cystic echinococcosis, caused by Echinococcus granulosus, is a neglected zoonosis that affects humans and livestock. This sero-survey was designed for the first time in Pakistan to assess the exposure of butchers to E. granulosus as there was no previous report in the country for this occupational group.

Methodology: Blood samples were collected from registered butchers (n = 364) in five different slaughterhouses in Faisalabad and Bahawalnagar Districts. Sera were tested for anti-Echinococcus granulosus IgG with a commercially available ELISA kit (specificity, 100%; sensitivity, 97%).

Results: Overall, seroprevalence was 9.61% (35/364). Butchers >30 years of age (10.34%), those involved in small ruminants butchery (11.70%), >10 years’ experience (10.04%), formal education level up to middle standard (10.28%), contact with dogs (12.71%), improper/unhygienic disposal of dog feces (11.87%), and those unaware of the consequences of eating with unwashed hands (13.80%) were more seropositive with significant statistical differences (p < 0.05). Variables like previous cyst encounter, no knowledge of zoonoses and/or cystic echinococcosis, living in rural areas and the presence of stray/feral dogs in surroundings did not show any significant association (p > 0.05) with seroprevalence in butchers. The binary logistic regression model also showed a statistically significant relationship (p < 0.05) for all risk factors found statistically significant (p < 0.05) in the univariate analysis.

Conclusions: This study shows high prevalence of cystic echinococcosis among butchers in Pakistan and underscores the need for educating native slaughterhouse personnel on cystic echinococcosis. It also serves as a global warning, especially in developing countries.

Published
2021-06-30
How to Cite
1.
Alvi MA, Li L, Saqib M, Ohiolei JA, Younas MW, Tayyab MH, Hassan A, Qamar W, Alvi AA, Fu B-Q, Yan H-B, Jia W-Z (2021) Serologic evidence of Echinococcus granulosus in slaughterhouses in Pakistan: global alarm for butchers in developing countries. J Infect Dev Ctries 15:861-869. doi: 10.3855/jidc.14029
Section
Original Articles

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