Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma

  • Zeinab Sayed Abdelkhalek Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
  • Mohamed Shehata Abdalla Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
  • Mona Mohamed Fathy Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
  • Tamer Mahmoud Elbaz Endemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, Egypt
  • Ashraf Omar Abdelaziz Endemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, Egypt
  • Mohamed Mahmoud Nabeel Endemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, Egypt
  • Hend Ibrahim Shousha Endemic medicine and Hepato-gastroenterology Department Faculty of Medicine, Cairo University, Cairo, Egypt
  • Amgad Hamed Kamel Department of Tropical medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt
  • Mai Hamed Kamel Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Keywords: Hepatocellular Carcinoma, diagnosis, microRNAs, TaqMan quantitative real-time reverse-transcription PCR

Abstract

Introduction: Several micro ribonucleic acids (miRNAs) are deregulated in hepatocellular carcinoma (HCC). Others are linked to clinical pathological features of HCC. The goal of this study was to investigate whether miRNA-21 and miRNA-215 gene expression could be used as a non-invasive diagnostic tool to diagnose HCC.

Methodology: The gene expression of mature miRNA -21 and miRNA -215 in serum was analysed retrospectively using singleplex TaqMan two-step stem-loop quantitative real-time reverse-transcription PCR in 40 patients with HCC, 40 with chronic hepatitis C virus (HCV) with cirrhosis and 40 apparently healthy controls.

Results: Expression of miRNA -21 was significantly more down regulated in patients with HCC than in those with non-cirrhotic HCV (P = 0.007; odds ratio = 5; 95% confidence interval 1.6–15.4). The receiver operating curve analysis of the ability of miRNA-21 expression to discriminate between HCC and non-cirrhotic HCV revealed an area under the curve of 0.712 with 70% sensitivity and 68% specificity at a cut-off of ≤ 1.4468. Thus, the expression level of miRNA -21 could discriminate HCC from non-cirrhotic HCV. Significant positive correlation was observed between expression levels of microRNA-21 and miRNA -215 (r = 0.783, p < 0.001), but no association was observed between expression level of miR-215 and HCC or chronic HCV (p = 0.474).

Conclusions: MiRNA-21 may be a useful, non-invasive tool for diagnosing HCC. Non-cirrhotic HCV patients have five times the risk of developing HCC when the miRNA -21 level ≤ 1.4468.

Published
2021-07-31
How to Cite
1.
Abdelkhalek ZS, Abdalla MS, Fathy MM, Elbaz TM, Abdelaziz AO, Nabeel MM, Shousha HI, Kamel AH, Kamel MH (2021) Role of circulating microRNA-21 and microRNA-215 in the diagnosis of hepatitis C related hepatocellular carcinoma. J Infect Dev Ctries 15:997-1003. doi: 10.3855/jidc.12230
Section
Original Articles