Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt

  • Samira Metwally Mohammad Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Magda Saad Ali Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Sara Ahmed Abdel-Rahman Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Raghda Abdelrahman Moustafa Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Mohamed Hassan Sarhan Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt http://orcid.org/0000-0002-5070-3922
Keywords: Children, COWP gene, Cryptosporidium, Genotyping, Modified Ziehl–Neelsen stain, PCR-RFLP

Abstract

Introduction: The protozoan parasite Cryptosporidium is one of the principal reasons for childhood diarrhea around the world. This work aimed to differentiate Cryptosporidium species among children suffering from diarrhea in Sharkyia Governorate, Egypt.

Methodology: A total of 97 fecal specimens were taken from children suffering from diarrhea, attending Pediatric Clinics of Zagazig University and Al-Ahrar Hospitals. Full history was taken. Stool samples were examined microscopically using modified Ziehl–Neelsen stain for detection of Cryptosporidium oocysts. To identify Cryptosporidium genotypes, positive samples were then subjected to nested Polymerase chain reaction-restriction fragment length polymorphism targeting Cryptosporidium oocyst wall protein gene.

Results: The overall detection rate was 27.8% (27/97) using modified Ziehl–Neelsen stain staining method. Using nested polymerase chain reaction, the gene was amplified in 85.2% (23/27). Restriction fragment length polymorphism analysis revealed that 65.2% (15/23) were Cryptosporidium hominis, 30.4% (7/23) were Cryptosporidium parvum, and one sample was not typed (4.4%). The significant risk factors associated with Cryptosporidium infection in children were animal contact and residence in rural areas.

Conclusions: Cryptosporidium is a common enteric parasite affecting children in Sharkyia Governorate, Egypt, with the predominance of C. hominis genotype in children.

Published
2021-10-31
How to Cite
1.
Mohammad SM, Ali MS, Abdel-Rahman SA, Moustafa RA, Sarhan MH (2021) Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt. J Infect Dev Ctries 15:1539-1546. doi: 10.3855/jidc.14367
Section
Original Articles