Draft genome sequence of first Vibrio diabolicus in Mexico strain InDRE-D1-M1, an emergent threat

  • Elena Berenice Nava-Soberanes Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Edgar Ruben Mendieta-Condado Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Fabiola Garcés-Ayala Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Adnan Araiza-Rodríguez Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Pablo Aguilera-Perez Department of Bacteriology, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Alberto Alfaro-López Department of Bacteriology, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Alma Cecilia Ibarra-Buelna Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Norma Angélica Montes-Colima Department of Bacteriology, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Irma López-Martínez Directorate of Diagnosis and Reference, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Lucía Hernández-Rivas Directorate of Services and Technical Support, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Afonso Méndez-Tenorio Laboratory of Biotechnology and Genomic Bioinformatics, National School of Biological Sciences (ENCB), National Polytechnic Institute, Mexico City, Mexico
  • Maribel González-Villa Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Jorge Membrillo-Hernandez Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • José Ernesto Ramírez-González Molecular Research and Development Unit, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
  • Alma Guadalupe Buelna-Romero Department of Bacteriology, Institute of Diagnostic and Epidemiological Reference "Dr. Manuel Martínez Báez" (InDRE), Ministry of Health, Mexico City, Mexico
Keywords: Vibrio diabolicus, environmental, pathogenicity, Mexico

Abstract

Introduction: The complete genome of the marine environmental bacterium Vibrio diabolicus isolated from raw shrimp in the city of Guadalajara in the state of Jalisco in Mexico is reported here.

Methodology: Vibrio spp. it was isolated and identified using standard microbiological and molecular techniques. Whole genome sequencing was performed using the Miseq system (Illumina, USA).

Results: The Multi Locus Sequence Typing profile of the isolated Vibrio bacteria coincided only with 4 specific loci (atpA, gyrB, pyrH and recA) and with a total coverage of the species belonging to Vibrio spp. Analysis of the complete genome of the Vibrio isolate and other closely related species, using the genomic fingerprints of the Virtual Analysis Method for PHylogenomic fingerprint estimation (VAMPHyRe) software, revealed the clustering of this species among the clade Vibrio diabolicus. The antibiogram revealed that this strain of Vibrio diabolicus is resistant to ampicillin, which is consistent with the bioinformatic finding of the β-lactamase enzyme that hydrolyzes carbenicillin class A.

Conclusions: This study demonstrated that the environmental marine bacterium Vibrio diabolicus contains carrier genes associated with pathogenicity and ecological function, which could represent a threat to public health.

Published
2021-11-30
How to Cite
1.
Nava-Soberanes EB, Mendieta-Condado ER, Garcés-Ayala F, Araiza-Rodríguez A, Aguilera-Perez P, Alfaro-López A, Ibarra-Buelna AC, Montes-Colima NA, López-Martínez I, Hernández-Rivas L, Méndez-Tenorio A, González-Villa M, Membrillo-Hernandez J, Ramírez-González JE, Buelna-Romero AG (2021) Draft genome sequence of first Vibrio diabolicus in Mexico strain InDRE-D1-M1, an emergent threat. J Infect Dev Ctries 15:1750-1754. doi: 10.3855/jidc.13233
Section
Brief Original Articles