Salmonella Schwarzengrund, Akuafo, and O:16 isolated from vacuum-packaged beef produced in the state of Mato Grosso, Brazil

  • Barbara Muller Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil
  • Adelino Cunha-Neto Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil https://orcid.org/0000-0003-4365-9502
  • Vinicius Castro Postgraduate program in Food Science, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil https://orcid.org/0000-0002-4746-0237
  • Ricardo Carvalho Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil https://orcid.org/0000-0002-0666-7135
  • Larrayane Carvalho Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil
  • Dalia Rodrigues National Reference Laboratory for Diagnosis of Enteric Bacteria, Oswaldo Cruz Fundation, Rio de Janeiro, Rio de Janeiro, Brazil https://orcid.org/0000-0003-1101-5518
  • Eduardo Eustáquio de Souza Figueiredo Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil https://orcid.org/0000-0002-2608-4324
Keywords: food production, Microbial contamination, Foodborne Illness, beef contamination, Beef exports

Abstract

Introduction: Salmonella spp. is a pathogen associated with foodborne infections, mainly in foods of animal origin. In this context, the present study investigated the occurrence of Salmonella serotypes, genotypes and the antimicrobial resistance profiles of strains in fresh beef produced in Mato Grosso, Brazil.

Methodology: A total of 107 samples from 13 different slaughterhouses in the Mato Grosso were analyzed. Suggestive Salmonella spp. colonies detected during the biochemical screening were submitted to DNA extraction, and hilA gene amplification was used for the PCR reaction. Antimicrobial resistance analyses were performed using 17 antimicrobial agents from eight different classes by the disk diffusion method. Strains exhibiting multiple drug resistances were submitted to PCR genotyping based on repetitive sequences (rep-PCR), using a commercial semiautomatic DiversiLab® system.

Results: A total of 5.6% (6/107) of the samples tested positive by the conventional method and were confirmed by PCR, namely two S. Akuafo, two non-typable Salmonella enterica strains, one Salmonella O:16 serovar, and one S. Schwarzengrund. The antimicrobial resistance profiles indicated resistance to gentamicin (30%), tetracycline, nitrofurantoin, and trimethoprim + sulfamethoxazole (16%). Genotyping indicated a 70% difference between S. Schwarzengrund and the non-typable Salmonella strains. No genetic similarities were observed between the six Salmonella isolates based on rep-PCR, including two S. Akuafo.

Conclusions: The results obtained herein corroborate that Salmonella serovar Schwarzengrund is commonly isolated in animal products in the state of Mato Grosso, Brazil, also highlighting the presence of two unusual Salmonella serovars in beef (Akuafo and O:16).

Author Biographies

Barbara Muller, Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil

Master Science student on Universidade Federal de Mato Grosso, postgraduate program in Food, Nutrition and Metabolism.

Adelino Cunha-Neto, Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil

Research Technichian at Federal University of Mato Grosso.

Vinicius Castro, Postgraduate program in Food Science, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil

BSc in Food Science (UFMT, 2015) MSc in Animal Science (UFMT, 2015-2017), PhD student in Food Science (UFRJ, 2017- currently). I am currently working on a project to investigate, by Molecular Biology, Shiga toxin-producing strains and other foodborne pathogens.

Ricardo Carvalho, Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil

Professor in Veterinary Medicine at Universidade de Cuiabá

Larrayane Carvalho, Postgraduate program in Nutrition, Food and Metabolism, Faculty of Nutrition, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil

Master Science student at Universidade Federal de Mato Grosso

Dalia Rodrigues, National Reference Laboratory for Diagnosis of Enteric Bacteria, Oswaldo Cruz Fundation, Rio de Janeiro, Rio de Janeiro, Brazil

Head of the Enterobacteria Laboratory at Oswaldo Cruz Institute

Published
2021-12-31
How to Cite
1.
Muller B, Cunha-NetoA, Castro VS, Carvalho RCT, Carvalho TeixeiraLA, RodriguesD dos P, de Souza FigueiredoEE (2021) Salmonella Schwarzengrund, Akuafo, and O:16 isolated from vacuum-packaged beef produced in the state of Mato Grosso, Brazil. J Infect Dev Ctries 15:1876-1882. doi: 10.3855/jidc.13726
Section
Original Articles