The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis

Azhar Iqbal; Muhammad Shafique; Muhammad Asif Zahoor; Saima Muzammil; Zeeshan Nawaz; Abdul Jabbar; Mohsin Khurshid; Riaz Hussain; Md. Akhttarul Islam; Ahmad Almatroudi; Khaled Saleh Allemailem; Muhammad Hidayat Rasool; Bilal Aslam

doi:10.3855/jidc.14990

The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis

Authors

Azhar Iqbal Department of Microbiology, Government College University Faisalabad, Pakistan
Muhammad Shafique Department of Microbiology, Government College University Faisalabad, Pakistan
Muhammad Asif Zahoor Department of Microbiology, Government College University Faisalabad, Pakistan
Saima Muzammil Department of Microbiology, Government College University Faisalabad, Pakistan
Zeeshan Nawaz Department of Microbiology, Government College University Faisalabad, Pakistan
Abdul Jabbar Department of Clinical Medicine, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Punjab, Pakistan
Mohsin Khurshid Department of Microbiology, Government College University Faisalabad, Pakistan
Riaz Hussain University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan
Md. Akhttarul Islam Statistics Discipline, Science, Engineering and Technology School, Khulna University Khulna, Bangladesh
Ahmad Almatroudi Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia
Khaled Saleh Allemailem Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia
Muhammad Hidayat Rasool Department of Microbiology, Government College University Faisalabad, Pakistan
Bilal Aslam Department of Microbiology, Government College University Faisalabad, Pakistan

DOI:

https://doi.org/10.3855/jidc.14990

Keywords:

Tuberculosis, MDR, TB, rifampicin, fluorescent microscopy, Pakistan

Abstract

Introduction: Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) is one of the leading causes of death in the world. The resource constraints make it difficult to diagnose and monitor the cases of MDR-TB. GeneXpert is a recognized tool used to diagnose the patients of pulmonary tuberculosis in clinical settings across the globe.

Methodology: The present one-year cross-sectional study was conducted to estimate the occurrence of MDR-TB in patients with pulmonary TB. A total of 1000 patients suspected of pulmonary tuberculosis were included in this study. A random convenient sampling technique was done to collect the sputum samples (twice) from the patients. Samples were processed for the detection of Mycobacterium tuberculosis using conventional detection methods like the Ziehl Nelson staining method and fluorescent microscopy. Additionally, Cepheid GeneXpert was used for molecular detection of MDR-TB in smear-positive samples of pulmonary tuberculosis by amplifying the rifampicin resistance determining region (RRDR; rpoB gene). All the tests were performed in the biosafety level III lab of District Headquarters Hospital Nankana Sahib.

Results: It was observed that 103 (10.3%) individuals were diagnosed as positive for tuberculosis among 1000 patients. Among these 103 TB positive cases, there were 11 (10.7%) patients diagnosed with rifampicin resistance gene (RR-Gene) of Mycobacterium tuberculosis.

Conclusions: Overall findings of the study showed that MDR-TB is prevalent in pulmonary TB patients and GeneXpert is the most sensitive technique for early diagnosis of the disease, which may be very helpful in the treatment and control of this public health menace in low and middle-income countries.

Author Biography

Bilal Aslam, Department of Microbiology, Government College University Faisalabad, Pakistan

Assistant professor, Department of Microbiology, Government College University, Faisalabad Pakistan

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Published

2022-04-30

How to Cite

Iqbal A, Shafique M, Zahoor MA, Muzammil S, Nawaz Z, Jabbar A, Khurshid M, Hussain R, Islam MA, Almatroudi A, Allemailem KS, Rasool MH, Aslam B (2022) The occurrence of multidrug-resistant Mycobacterium tuberculosis from patients of pulmonary tuberculosis. J Infect Dev Ctries 16:698–704. doi: 10.3855/jidc.14990

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Issue

Vol. 16 No. 04: April 2022

Section

Original Articles

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