In vitro antifungal activity of Morinda citrifolia (noni) extract against Candida albicans

  • Camila Botelho Miguel Unidade de Biociências, Centro Universitário de Mineiros, Mineiros, GO, Brazil
  • Renato Ventresqui Oliveira Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
  • Wellington Francisco Rodrigues Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Gustavo Tavares Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Sandro Calderano Joinhas Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Maria Auxiliadora Gomes da Cruz Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Célia Fabrício de Souza Rezende Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Andréa Maria Pasquini Alvarenga Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Weder da Silva Mateus Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
  • Marcia Laina Silveira Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
  • Elizabeth Uber Bucek Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Weslley Guimarães Bovi Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Carlo José Freire de Oliveira Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Thiago Alvares da Costa Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Javier Emilio Lazo-Chica Laboratório de Biologia Celular, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Tony de Paiva Paulino Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
Keywords: Morinda citrifolia, noni, Candida albicans, THP-1, HeLa, cytotoxicity

Abstract

Introduction: Candida albicans is the main agent of the most common fungal infection, Candidiasis. It is an opportunistic and dangerous pathogen, especially in immunosuppressed patients. The biological properties of Morinda citrifolia (noni) make it a potent antifungal. In this study, antifungal effect of M. citrifolia was evaluated to verify its effect on human cells.

Methodology: Extract of M. citrifolia was used against strains of C. albicans (cEC 1291). Glucose consumption in C. albicans biofilm was determined at different concentrations of M. citrifolia, and germ tube formation was evaluated in the presence and absence of M. citrifolia. Fungicidal activity was determined by the kinetics of fungal cell death. THP-1 and HeLa cells were used for cell viability and apoptosis, and cell proliferation assays, respectively.

Results: Cells treated with M. citrifolia maintained higher concentration of glucose than the control group (p < 0.05). Germ tube formation was inhibited in cells treated with M. citrifolia (p < 0.05). M. citrifolia exerted a cytotoxic effect on C. albicans cells with 99.99% lethality after 6.82 h (1:1 and 1:2), and reduced the viability of THP-1 cells by 25% and 67% after 12 and 36 h, respectively. Annexin V expression in THP-1 increased in groups that received higher concentrations of M. citrifolia (p < 0.05), reducing the proliferation of THP-1 and HeLa cells (2.8-fold). A greater cytotoxic effect was observed in fungal cells.

Conclusions: These results indicate that M. citrifolia exerts biological activity against C. albicans and reduces the viability and proliferation of human cells.

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Published
2022-07-28
How to Cite
1.
Miguel CB, Oliveira RV, Rodrigues WF, Tavares G, Joinhas SC, Gomes da CruzMA, RezendeCF de S, Alvarenga AMP, MateusW da S, Silveira ML, Bucek EU, Bovi WG, de OliveiraCJF, da CostaTA, Lazo-ChicaJE, PaulinoT de P (2022) In vitro antifungal activity of Morinda citrifolia (noni) extract against Candida albicans. J Infect Dev Ctries 16:1206-1217. doi: 10.3855/jidc.15835
Section
Original Articles