In vitro antifungal activity of Morinda citrifolia (noni) extract against Candida albicans

Authors

  • Camila Botelho Miguel Unidade de Biociências, Centro Universitário de Mineiros, Mineiros, GO, Brazil
  • Renato Ventresqui Oliveira Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
  • Wellington Francisco Rodrigues Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Gustavo Tavares Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Sandro Calderano Joinhas Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Maria Auxiliadora Gomes da Cruz Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Célia Fabrício de Souza Rezende Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Andréa Maria Pasquini Alvarenga Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Weder da Silva Mateus Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
  • Marcia Laina Silveira Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil
  • Elizabeth Uber Bucek Laboratório de Ciências Farmacêuticas, Universidade de Uberaba, Uberaba, MG, Brazil
  • Weslley Guimarães Bovi Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Carlo José Freire de Oliveira Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Thiago Alvares da Costa Curso de Pós-graduação em Medicina Tropical e Infectologia, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Javier Emilio Lazo-Chica Laboratório de Biologia Celular, Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, MG, Brazil
  • Tony de Paiva Paulino Laboratório de Pesquisas do Cefores, Universidade Federal do Triângulo Mineiro, Praça Manoel Terra, Uberaba, MG, Brazil

DOI:

https://doi.org/10.3855/jidc.15835

Keywords:

Morinda citrifolia, noni, Candida albicans, THP-1, HeLa, cytotoxicity

Abstract

Introduction: Candida albicans is the main agent of the most common fungal infection, Candidiasis. It is an opportunistic and dangerous pathogen, especially in immunosuppressed patients. The biological properties of Morinda citrifolia (noni) make it a potent antifungal. In this study, antifungal effect of M. citrifolia was evaluated to verify its effect on human cells.

Methodology: Extract of M. citrifolia was used against strains of C. albicans (cEC 1291). Glucose consumption in C. albicans biofilm was determined at different concentrations of M. citrifolia, and germ tube formation was evaluated in the presence and absence of M. citrifolia. Fungicidal activity was determined by the kinetics of fungal cell death. THP-1 and HeLa cells were used for cell viability and apoptosis, and cell proliferation assays, respectively.

Results: Cells treated with M. citrifolia maintained higher concentration of glucose than the control group (p < 0.05). Germ tube formation was inhibited in cells treated with M. citrifolia (p < 0.05). M. citrifolia exerted a cytotoxic effect on C. albicans cells with 99.99% lethality after 6.82 h (1:1 and 1:2), and reduced the viability of THP-1 cells by 25% and 67% after 12 and 36 h, respectively. Annexin V expression in THP-1 increased in groups that received higher concentrations of M. citrifolia (p < 0.05), reducing the proliferation of THP-1 and HeLa cells (2.8-fold). A greater cytotoxic effect was observed in fungal cells.

Conclusions: These results indicate that M. citrifolia exerts biological activity against C. albicans and reduces the viability and proliferation of human cells.

Downloads

Published

2022-07-28

How to Cite

1.
Miguel CB, Oliveira RV, Rodrigues WF, Tavares G, Joinhas SC, Gomes da Cruz MA, Rezende CF de S, Alvarenga AMP, Mateus W da S, Silveira ML, Bucek EU, Bovi WG, de Oliveira CJF, da Costa TA, Lazo-Chica JE, Paulino T de P (2022) In vitro antifungal activity of Morinda citrifolia (noni) extract against Candida albicans. J Infect Dev Ctries 16:1206–1217. doi: 10.3855/jidc.15835

Issue

Vol. 16 No. 07: July 2022

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC