Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources

Authors

  • Leonardo Moreira Lima Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0001-9983-4600
  • Gustavo Perdoncini Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-3249-5701
  • Karen Apellanis Borges Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0001-6649-5833
  • Thales Quedi Furian Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0003-0376-8616
  • Carlos Tadeu Pippi Salle Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-0286-7148
  • Hamilton Luiz de Souza Moraes Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
  • Vladimir Pinheiro do Nascimento Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil https://orcid.org/0000-0002-7720-3274

DOI:

https://doi.org/10.3855/jidc.16485

Keywords:

Broiler, Campylobacteriosis, Campylobacter jejuni, human, virulence marker

Abstract

Introduction: Campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis. Despite its public health importance, the virulence factors and mechanisms underlying C. jejuni pathogenesis remain poorly understood and the relationships between these genes and the sources of the strains are not clear. We aimed to determine the virulence profiles of C. jejuni isolated from poultry and human cases of Campylobacteriosis.

Methodology: A total of 50 strains of C. jejuni isolated from poultry and human cases of Campylobacteriosis were screened by polymerase chain reaction (PCR) for the presence of six pathogenic genes (flaA, iam, wlaN, cdtA, cdtB, cdtC).

Results: A total of 40% (10/25) of the human isolates presented only one virulence marker. In contrast, 64% (16/25) of the poultry-derived strains showed four or five virulence markers. cdtA and flaA occurred more frequently in poultry-derived strains than in human strains. Ten different virulence profiles were observed among the human isolates and 11 among the poultry strains. Only four profiles were common to both sources: profiles 3 (flaA, cdtA, cdtB, and cdtC), 5 (cdtA and cdtB), 7 (flaA and cdtB), and 10 (iam, flaA, cdtA, cdtB, and cdtC). The human-derived strains had a higher Shannon diversity index (1.9396) and Simpson index (0.8367), indicating a more diversified population than found in poultry (1.7742 and 0.7333, respectively).

Conclusions: We found variations in the genetic profiles of the circulating strains based on the isolation source and genes that are potentially pathogenic to humans were detected in poultry-derived strains.

Author Biography

Hamilton Luiz de Souza Moraes, Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

Centro de Diagnóstico e Pesquisa em Patologia Aviária

Departamento de Medicina Animal

Faculdade de Veterinária

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Published

2022-09-30

How to Cite

1.
Moreira Lima L, Perdoncini G, Apellanis Borges K, Quedi Furian T, Pippi Salle CT, de Souza Moraes HL, Pinheiro do Nascimento V (2022) Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources. J Infect Dev Ctries 16:1466–1472. doi: 10.3855/jidc.16485

Issue

Vol. 16 No. 09: September 2022

Section

Original Articles

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