Detection and antibiotic resistance of diarrheagenic Escherichia coli from patients with diarrhea in Ulaanbaatar, Mongolia

Munkhdelger Yandag; Altansukh Tsend-Ayush; Nyamaa Gunregjav; Otgontsetseg Erdenebayar; Bayarlakh Byambadorj; Nishi Juniichiro; Sarantuya Jav

doi:10.3855/jidc.17256

Detection and antibiotic resistance of diarrheagenic Escherichia coli from patients with diarrhea in Ulaanbaatar, Mongolia

Authors

Munkhdelger Yandag Department of Molecular Biology and Genetics, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia https://orcid.org/0000-0003-0985-3491
Altansukh Tsend-Ayush Department of Molecular Biology and Genetics, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia https://orcid.org/0000-0003-4570-5998
Nyamaa Gunregjav National Center for Communicable Diseases, Ulaanbaatar, Mongolia
Otgontsetseg Erdenebayar Department of Molecular Biology and Genetics, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia https://orcid.org/0000-0002-1632-4670
Bayarlakh Byambadorj Department of Molecular Biology and Genetics, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia https://orcid.org/0000-0002-8164-681X
Nishi Juniichiro Department of Microbiology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan https://orcid.org/0000-0002-2839-9434
Sarantuya Jav Department of Molecular Biology and Genetics, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

DOI:

https://doi.org/10.3855/jidc.17256

Keywords:

Mongolia, diarrheagenic Escherichia coli, HEp-2 cell adherence assay, multiplex PCR

Abstract

Introduction: Diarrheal diseases are common with worldwide distribution, and diarrheagenic Escherichia coli (DEC) strains are the main causative agents. The present study aimed to define the association of various pathotypes of E. coli from diarrheal patients in Mongolia.

Methodology: A total of 341 E. coli strains were isolated from the stool of diarrheal patients. Bacterial susceptibility to antimicrobial agents was determined by the Kirby Bauer disk diffusion method. DEC isolates were identified by HEp-2 cell adherence assay and multiplex polymerase chain reaction (PCR).

Results: DEC pathogens were detected in 53.7% of 341 E. coli isolates. Enteroaggregative E. coli (EAEC) was the most common DEC pathotype identified by HEp-2 adherence assay and multiplex PCR methods in 97 samples (28.4%), followed by atypical enteropathogenic E. coli (EPEC) in 50 samples (14.7%), diffusely adherent E. coli (DAEC) in 25 samples (7.3%), enterohaemorrhagic E. coli (EHEC) in 6 samples (1.8%), enterotoxigenic E. coli (ETEC) in 4 samples (1.2%), and enteroinvasive E. coli (EIEC) in 1 sample (0.3%). DEC strains had > 50% antibiotic resistance against cephalothin, ampicillin, and trimethoprim/sulfamethoxazole. All tested DEC strains were susceptible to imipenem. Among the 183 DEC strains, 27 (14.8%) were extended spectrum beta-lactamase producing isolates, and 125 (68.3%) isolates were multiple drug resistant.

Conclusions: We have identified six pathotypes of DEC from the clinical isolates tested and concluded that a high prevalence of antimicrobial resistance was observed in these pathotypes. EAEC was the most common pathotype identified and this is the first report of EHEC identification in Mongolia.

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Published

2023-02-28

How to Cite

Yandag M, Tsend-Ayush A, Gunregjav N, Erdenebayar O, Byambadorj B, Juniichiro N, Jav S (2023) Detection and antibiotic resistance of diarrheagenic Escherichia coli from patients with diarrhea in Ulaanbaatar, Mongolia. J Infect Dev Ctries 17:202–209. doi: 10.3855/jidc.17256

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Issue

Vol. 17 No. 02: February 2023

Section

Original Articles

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