Characterization of carbapenemase-producing Gram-negative bacilli: first report of blaNDM-1 in Enterobacter cloacae

Manel Debabza; Raoudha Dziri; Abdelbasset Mechai; Amel Bouguessa; Naouel Klibi; Hadda Imene Ouzari

doi:10.3855/jidc.18031

Characterization of carbapenemase-producing Gram-negative bacilli: first report of blaNDM-1 in Enterobacter cloacae

Authors

Manel Debabza Bioactive Molecules and Applications Laboratory, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University (E.C.L.T.U)-Tebessa, Algeria https://orcid.org/0000-0002-5821-7697
Raoudha Dziri Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, El-Manar II, Tunisia https://orcid.org/0000-0003-1783-5672
Abdelbasset Mechai Bioactive Molecules and Applications Laboratory, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University (E.C.L.T.U)-Tebessa, Algeria https://orcid.org/0000-0003-1241-3921
Amel Bouguessa Microbiology Laboratory, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University (E.C.L.T.U)-Tebessa, Algeria
Naouel Klibi Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, El-Manar II, Tunisia
Hadda Imene Ouzari Laboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El Manar, El-Manar II, Tunisia https://orcid.org/0000-0002-3237-554X

DOI:

https://doi.org/10.3855/jidc.18031

Keywords:

Gram-negative bacilli, Extended-spectrum β-lactamases, Carbapenemases, Metallo-beta-lactamases, Enterobacter cloacae, blaNDM-1

Abstract

Introduction: The spread of multidrug-resistant bacteria, particularly carbapenem-resistant Gram-negative bacilli (CR-GNB), has become a serious challenge for clinicians due to limited therapeutic options. The aim of the study was to investigate the prevalence of carbapenemase production among clinical isolates recovered from 352 samples collected in Tebessa hospital, Algeria.

Methodology: Bacterial isolates were identified by 16S RNA gene sequencing and susceptibility to antibiotics was determined by disk diffusion method. Carbapenem-resistant isolates were screened for carbapenemase production using modified carba Nordmann-Poirel test, modified Hodge test and imipenem-EDTA combined disc test. Extended-spectrum β-lactamases (ESBL) were detected using double-disk synergy test. Molecular characterization of carbapenemases and ESBL genes was performed by polymerase chain reaction (PCR) and sequencing.

Results: A total of 85 Gram-negative bacilli isolates were recovered mainly from urine samples and were identified as: Klebsiella pneumoniae (17.65%), Serratia odorifera (15.29%), Escherichia coli (12.94%), Raoultella ornithinolytica, Enterobacter cloacae (11.76%), Serratia marcescens (10.59%), Morganella morganii (7.06%), Proteus mirabilis (5.88%), Acinetobacter baumannii (4.70%) and Pseudomonas aeruginosa (2.35%). All strains were resistant or intermediate to imipenem and/or ertapenem. ESBL, carbapenemase and metallo-beta-lactamases (MBL) phenotypes were detected in 19 (22.35%), 9 (10.59%) and 2 (2.35%) GNB isolates, respectively. PCR results in nine carbapenemase-producing GNB strains chosen showed the presence of one to four carbapenemase genes (bla_GES, bla_SME, bla_NDM-1, bla_VIM, bla_GIM, bla_SPM, bla_OXA-48) in four strains; however, seven strains had at least one ESBL gene (bla_TEM-1, bla_CTXM-15, bla_SHV).

Conclusions: In this study, we report the first incidence of bla_NDM-1 gene in Enterobacter cloacae isolated from urine sample in Algeria.

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Published

2023-09-30

How to Cite

Debabza M, Dziri R, Mechai A, Bouguessa A, Klibi N, Ouzari HI (2023) Characterization of carbapenemase-producing Gram-negative bacilli: first report of blaNDM-1 in Enterobacter cloacae. J Infect Dev Ctries 17:1300–1309. doi: 10.3855/jidc.18031

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Issue

Vol. 17 No. 09: September 2023

Section

Original Articles

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