In silico identification of cytotoxic T lymphocyte epitopes encoded by RD5 region of Mycobacterium tuberculosis

  • Jun-Wei Zhao Zhengzhou University, Zhengzhou, Henan, China
  • Min Yan Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China
  • Guang Shi Zhengzhou University, Zhengzhou, Henan, China
  • Shu-Lin Zhang Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • Liang Ming Zhengzhou University, Zhengzhou, Henan, China

Abstract

Introduction: While CD8+ T cells (cytotoxic T lymphocytes, CTLs) play important roles in immunity against Mycobacterium tuberculosis, only a small number of human leukocyte differentiation antigen (HLA) class I-restricted CTL epitopes for TB have been identified. The current study evaluates CTL epitopes of Rv3117 and Rv3120 proteins, two newly found M. tuberculosis region-diffference-5 (RD5)-encoded antigens, and their population coverage.


Methodology: The amino acid sequences of the two proteins were subjected to epitope analysis under HLA-A2, A3 and B7 supertype restriction using NetCTL, SYFPEITHI, BIMAS, NetCTLPan, IEDB, NetMHC and NetMHCPan prediction online servers.


Results: Eight RD5-encoded CTL epitopes were identified in the two proteins and the average population coverage of these epitopes was 87.2% among populations worldwide.


Conclusion: These CTL epitopes that were identified in silico and may have potential use for CD8+ T cell-mediated TB vaccine design.

Published
2017-11-06
How to Cite
Zhao J, Yan M, Shi G, Zhang S, Ming L (2017) In silico identification of cytotoxic T lymphocyte epitopes encoded by RD5 region of Mycobacterium tuberculosis. The Journal Of Infection In Developing Countries 11 (10): 806-810. https://doi.org/10.3855/jidc.7207
Section
Brief Original Articles

Keywords

Tuberculosis; region of difference 5; cytotoxic T lymphocyte epitope; population coverage