The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria

  • Joseph Gangoue-Pieboji Centre for Proteins Engineering, University of Liège, Institute of Chemistry B6, Sart-Tilman, B 4000 Liège
  • Noelly Eze Laboratory of General Biology, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé
  • Arnaud Ngongang Djintchui Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé
  • Bathélémy Ngameni Department of Pharmacy and Traditional Pharmacopoeia, Faculty of Medicine and Biomedical Science, University of Yaoundé I, P.O. Box 8664, Yaoundé
  • Nolé Tsabang Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plant Studies, P.O. Box 8404, Yaoundé
  • Dieudonné Emmanuel Pegnyemb Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon
  • Lucie Biyiti Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plant Studies
  • Pierre Ngassam Laboratory of General Biology, Faculty of Science, University of Yaoundé I, P.O. Box 812 Yaoundé
  • Sinata Koulla-Shiro Department of Microbiology, Parasitology, Hematology and infectious Diseases, Faculty of Medicine and Biomedical Science, University of Yaoundé I, P.O. Box 1364, Yaoundé
  • Moreno Galleni Centre for Proteins Engineering, University of Liège, Institute of Chemistry B6, Sart-Tilman, B 4000 Liège
Keywords: β-lactam-resistant bacteria, antimicrobial activity, Cameroon, Medicinal plant, β-lactamase

Abstract

Background: In effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria.

Methodology: The antimicrobial activities of plant extracts were evaluated against clinically proved beta-lactam-resistant bacteria (Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Serratia marcescens, Acinetobacter baumannii, Staphylococcus aureus and Enterococcus sp.) and reference strains of bacteria (Escherichia coli ATCC 35218, Enterobacter aerogenes ATCC 29751, E. aerogenes ATCC 13048, Pseudomonas aeruginosa ATCC 27853 and Enterococcus hirae ATCC 9790) by using disc-diffusion and agar-dilution assays.

Results: The crude plant extracts demonstrated broad spectrum activity against all bacteria tested with inhibition zones in the range of 8-30 mm. The minimal inhibitory concentration (MIC) values of different plant extracts against the tested bacteria were found to range from ≤ 0.3 to ≥ 10 mg ml-1. The most active plant extracts were from Dortenia picta and Bridelia micrantha (MIC: 1.25-10 mg ml-1) on beta-lactam-resistant Gram-negative bacilli and the extracts from B. micrantha, Mallotus oppositifolius, Garcinia lucida, Garcinia. kola, Campylospermum densiflorum (leaves) and C. zenkeri (root) on beta-lactam-resistant Gram-positive cocci (MIC: ≤ 0.3-5 mg ml-1).

Conclusion: Of the 17 plant extracts studied, seven showed good antimicrobial activity against the tested bacteria. The stem bark of B. micrantha and the leaves of D. picta were most active towards beta-lactamase producing Gram-negative bacilli. This study shows that medicinal plants could be sources of compounds which can be used to fight against beta-lactam resistant bacteria.
Published
2009-10-22
How to Cite
1.
Gangoue-Pieboji J, Eze N, Ngongang Djintchui A, Ngameni B, Tsabang N, Pegnyemb DE, Biyiti L, Ngassam P, Koulla-Shiro S, Galleni M (2009) The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria. J Infect Dev Ctries 3:671-680. doi: 10.3855/jidc.77
Section
Original Articles