Evaluation of in-house loop-mediated isothermal amplification for tuberculosis diagnosis compared with Xpert MTB/RIF

  • Charity Habeenzu University Teaching Hospital, Ministry of Health, Lusaka, Zambia
  • Chie Nakajima Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan
  • Eddie Solo University Teaching Hospital, Ministry of Health, Lusaka, Zambia
  • Precious Bwalya University Teaching Hospital, Ministry of Health, Lusaka, Zambia
  • Kiichi Kajino Hokkaido University, Sapporo, Japan
  • Mari Miller University Teaching Hospital, Ministry of Health, Lusaka, Zambia
  • Youichi Kurosawa Tottori University Faculty of Medicine, Tottori, Japan
  • Victor Mudenda University Teaching Hospital, Ministry of Health, Lusaka, Zambia
  • Lackson Kasonka University Teaching Hospital, Ministry of Health, Lusaka, Zambia
  • Yasuhiko Suzuki Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan
  • Takashi Matsuba Tottori University Faculty of Medicine, Tottori, Japan

Abstract

Introduction: To evaluate the diagnostic performances of an in-house loop-mediated isothermal amplification (LAMP) kit and the Xpert MTB/RIF test for the diagnosis of pulmonary tuberculosis in a resource-limited setting, this study was performed at the University Teaching Hospital, Ministry of Health, the Republic of Zambia.


Methodology: Two hundred sputum specimens obtained from new tuberculosis (TB) suspects were used for the evaluation of the diagnostic performance of an in-house LAMP kit in comparison with the Xpert MTB/RIF kit.


Results: The sensitivity of in-house LAMP and Xpert MTB/RIF was 96.9% and 95.4% in smear-positive samples, 96.8% and 100% in smear-positive/culture-positive samples, and 39.1% and 73.9% in smear-negative/culture-positive samples, respectively. The specificity of in-house LAMP and MTB/RIF kits with culture was 96.5% and 94.5%, respectively. This indicated the superiority of the Xpert MTB/RIF kit; however, mechanical errors during sample processing and the insufficient quantity of samples by Xpert MTB/RIF kit occurred at 2.0% and 19.7%, respectively, comparing to the 100% accessibility of in-house LAMP.


Conclusions: Considering the results obtained in this study together with the easy setup with much simpler equipment, such as an aluminum heat block or water bath, in in-house LAMP compared with real-time polymerase chain reaction equipment in Xpert MTB/RIF kit, the applicability of in-house LAMP for the screening of tuberculosis directly from sputum in resource-limited setting seemed to be high.

Author Biographies

Charity Habeenzu, University Teaching Hospital, Ministry of Health, Lusaka, Zambia

Department of Microbiology and Pathology

Chie Nakajima, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan

Global station for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan

Eddie Solo, University Teaching Hospital, Ministry of Health, Lusaka, Zambia

Department of Microbiology and Pathology

Precious Bwalya, University Teaching Hospital, Ministry of Health, Lusaka, Zambia

Department of Microbiology and Pathology

Kiichi Kajino, Hokkaido University, Sapporo, Japan

Global station for Zoonosis Control, Global Institution for Collaborative Research and Education

Mari Miller, University Teaching Hospital, Ministry of Health, Lusaka, Zambia

Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan

Youichi Kurosawa, Tottori University Faculty of Medicine, Tottori, Japan

Division of Health Administration and Promotion, Department of Social Medicine

Victor Mudenda, University Teaching Hospital, Ministry of Health, Lusaka, Zambia

Department of Microbiology and Pathology

Yasuhiko Suzuki, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan

Global station for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan

Takashi Matsuba, Tottori University Faculty of Medicine, Tottori, Japan

Division of Bacteriology, Department of Microbiology and Immunology

Published
2017-06-27
How to Cite
Habeenzu C, Nakajima C, Solo E, Bwalya P, Kajino K, Miller M, Kurosawa Y, Mudenda V, Kasonka L, Suzuki Y, Matsuba T (2017) Evaluation of in-house loop-mediated isothermal amplification for tuberculosis diagnosis compared with Xpert MTB/RIF. The Journal Of Infection In Developing Countries 11 (06): 440-444. https://doi.org/10.3855/jidc.7730
Section
Original Articles

Keywords

in-house LAMP; Xpert; tuberculosis