Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids

Microbiological Characteristics of hvKP Isolates

  • Xiumei Xiao Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China
  • Bei Yao Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China
  • Qingtao Zhou Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China
  • Jie Zhang Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China

Abstract

Introduction: Reports of hypermucoviscous Klebsiella pneumoniae (hvKP) isolated from fluids other than blood or abscess are rare. The aim of the study was to compare clinical and microbiological characteristics of hvKP found in blood or abscess fluid with those isolated from other loci.


Methodology: A total of 24 non-repetitive hvKP isolates were collected from January 2013 to June 2014 from patients with hvKP infections. There were 15 in Group 1 (fluid other than blood or abscess) and 9 in Group 2 (blood or abscess fluid). Medical records of all patients were reviewed. Capsular polysaccharide (CPS) typing, virulence factor determination, and multilocus sequence typing (MLST) of hvKP isolates were performed.


Results: Seventeen sequence types (STs) and 6 capsular serotypes were identified. Type K2CC65 was most commonly identified in Group 1 and type K2CC86 in Group 2. Deletion of pLVPK-derived loci were found in K2 and non-K1/K2 hvKP strains. Two virulent genes, fimH and ycfM, were identified more frequently in Group 2 than in Group 1. There was no difference in the frequency of other virulent genes or serotypes in the two groups. Two imipenem resistant hvKP isolates (cr-hvKP) were found in non-blood or abscess samples.


Conclusions: hvKP isolated from different body fluids had similar clinical and microbiological characteristics. cr-hvKP identified in non-blood or abscess samples should raise our attention to the challenging situation and management of hvKP infection.

Author Biographies

Xiumei Xiao, Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China

Department of Laboratory Medicine

Bei Yao, Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China

Department of Laboratory Medicine

Qingtao Zhou, Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China

Department of Respiratory Medicine

Jie Zhang, Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China

Department of Laboratory Medicine

Published
2017-10-07
How to Cite
Xiao X, Yao B, Zhou Q, Zhang J (2017) Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids. The Journal Of Infection In Developing Countries 11 (09): 672-678. https://doi.org/10.3855/jidc.8132
Section
Emerging Problems in Infectious Diseases

Keywords

hypermucoviscous Klebsiella pneumoniae, microbiological characteristics, virulence, non-blood/abscess sample