Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA

  • Sibel Aydogan Ankara Ataturk Training and Research Hospital, Ankara, Turkey
  • Ziya Cibal Acikgoz Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
  • Aysegul Gozalan Ankara Ataturk Training and Research Hospital, Ankara, Turkey
  • Fisun Kirca Ankara Ataturk Training and Research Hospital, Ankara, Turkey
  • Tuba Muderris Ankara Ataturk Training and Research Hospital, Ankara, Turkey
  • Elife Ozturk Ankara Ataturk Training and Research Hospital, Ankara, Turkey

Abstract

Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections.


Methodology: The study was conducted on 121 and 54 clinical samples for the detection of HBV DNA and HCV RNA, respectively, with an additional 8 HCV RNA external quality control samples.


Results: Though a high correlation was observed between the two kits for the HBV DNA (r = 0.955, p = 0.001) and HCV RNA quantifications (r = 0.828, p = 0.001), discordant results were found in nine of the HBV DNA and in six of the HCV RNA samples. The mean difference between the two systems was found to be 0.4 log IU/mL in Quality Control for Molecular Diagnostics (QCMD) HCV RNA samples by Bland-Altman analysis.


Conclusions: Although there was a high correlation between HBV DNA and HCV RNA tests according to the results of the study, the RTA system requires improvement for the determination of HCV RNA.

Author Biographies

Sibel Aydogan, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

Department of Medical Microbiology

Ziya Cibal Acikgoz, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey

Department of Medical Microbiology, Faculty of Medicine

Aysegul Gozalan, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

Department of Medical Microbiology

Fisun Kirca, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

Department of Medical Microbiology

Tuba Muderris, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

Department of Medical Microbiology

Elife Ozturk, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

Molecular Genetics Laboratory

Published
2017-07-31
How to Cite
Aydogan S, Acikgoz Z, Gozalan A, Kirca F, Muderris T, Ozturk E (2017) Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA. The Journal Of Infection In Developing Countries 11 (07): 543-548. https://doi.org/10.3855/jidc.8363
Section
Original Articles

Keywords

Artus RG, RTA, real time PCR, spin column, viral hepatitis