Mycobacterium tuberculosis strains from ancient and modern lineages induce distinct patterns of immune responses

  • Pampi Chakraborty St. Xavier’s College, Mumbai, India
  • Savita Kulkarni Bhabha Atomic Research Centre, TMH Annexe, Parel, Mumbai, India
  • Ramakrishna Rajan Bhabha Atomic Research Centre, Modular Lab, Trombay, Mumbai, India
  • Krishna Sainis Bhabha Atomic Research Centre, Modular Lab, Trombay, Mumbai, India
Keywords: Mycobacterium tuberculosis, lineage, immune response

Abstract

Introduction: It is possible that the difference in virulence and prevalence of different strains of Mycobacterium tuberculosis is related to the diverse immune response they evoke in the host. Outbreak strains have been shown to subvert the innate immune response as a potential host evasion mechanism. However, the immunological outcome of the interactions of different clinical strains with different host cells is still not understood.

Methodology: Extremely Drug Resistant (XDR) Beijing, a modern lineage clinical strain and a comparator ancient lineage strain, EAI-5, were selected for the present study. The early induction of proinflammatory cytokines in human peripheral blood monocyte derived macrophages (MDM), monocyte derived dendritic cells (MDDC) and whole blood (WB) infected by selected clinical isolates and laboratory strains H37Rv and BCG were assessed.

Results: The two clinical strains exhibited distinct patterns of cytokine induction. The ancient lineage strain induced substantially higher expression of all proinflammatory cytokines like TNF-α, IL-1β, IL-12 and chemokines like MCP-1, IL-8. However, the modern lineage strain exhibited suppressed response for the same. Further, the immune responses to two strains were conserved in infected MDM, MDDC and WB i.e. showing similar patterns of response across multiple human hosts. However, the differential response pattern was not observed when bacterial sonicates were used instead of live mycobacteria.

Conclusions: The lineage specific patterns in induction of proinflammatory cytokines and chemokines by different M. tuberculosis strains remain similar in macrophage and dendritic cells isolated from different individuals. The present study also confirms that whole cell sonicates of different lineages of M. tuberculosis fail to induce such lineage specific response.

Author Biographies

Pampi Chakraborty, St. Xavier’s College, Mumbai, India

Department of Microbiology, Assistant professor

Savita Kulkarni, Bhabha Atomic Research Centre, TMH Annexe, Parel, Mumbai, India

Radiation Medicine Centre, Scientific Officer (G), Head Tuberculosis Immunology & Immunoassay Development Section

Ramakrishna Rajan, Bhabha Atomic Research Centre, Modular Lab, Trombay, Mumbai, India

Raja Ramanna Fellow, Bhabha Atomic Research Centre

Krishna Sainis, Bhabha Atomic Research Centre, Modular Lab, Trombay, Mumbai, India

Raja Ramanna Fellow, Bhabha Atomic Research Centre

Published
2018-01-10
How to Cite
1.
Chakraborty P, Kulkarni S, Rajan R, Sainis K (2018) Mycobacterium tuberculosis strains from ancient and modern lineages induce distinct patterns of immune responses. J Infect Dev Ctries 11:904-911. doi: https://doi.org/10.3855/jidc.8596
Section
Original Articles