Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters

  • Farzaneh Firoozeh School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran
  • Leila Soleimani-Moorchekhorti School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran
  • Mohammad Zibaei School of Medicine, Alborz University of Medical Sciences, Karaj, I.R. Iran

Abstract

Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs). The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters.


Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536, II 536, III 536, IV536, I CFT073, II CFT073, PAI IJ96, and PAI IIJ96).


Results: Of 78 patients with a UTI 31 (39.7%) used indwelling catheters. Of these 31 patients, 27 (87.1%) carried PAIs markers, including 25 (80.6%) PAI IV536, 12 (38.7%) PAI ICFT073, 6 (19.4%) PAI IICFT073, 6 (19.4%) PAI III536 and 3 (9.7%) PAI II536. PAI I536, PAI IJ96, and PAI IIJ96 were not detected in the UPEC strains.


Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.

Author Biographies

Farzaneh Firoozeh, School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran

Department of Microbiology & Immunology,
School of Medicine

Leila Soleimani-Moorchekhorti, School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. Iran

Department of Microbiology & Immunology,
School of Medicine

Mohammad Zibaei, School of Medicine, Alborz University of Medical Sciences, Karaj, I.R. Iran

Department of Parasitology and Mycology,
School of Medicine

Published
2017-07-31
How to Cite
Firoozeh F, Soleimani-Moorchekhorti L, Zibaei M (2017) Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters. The Journal Of Infection In Developing Countries 11 (07): 557-562. https://doi.org/10.3855/jidc.8660
Section
Original Articles

Keywords

pathogenicity islands, Uropathogenic, Escherichia coli, urinary catheter