Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance

Finding of Salmonella on pig carcasses in Serbia

  • Jasna Mirko Kureljusic Institute of Veterinary Medicine of Serbia, Belgrade, Serbia
  • Marko Predrag Dmitrić Veterinary Specialized Institute “Kraljevo”, Kraljevo, Serbia
  • Dejan Svetislav Vidanović Veterinary Specialized Institute “Kraljevo“, Kraljevo, Serbia
  • Vlado Božidar Teodorović Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
  • Branislav Ilija Kureljušić Institute of Veterinary Medicine of Serbia, Belgrade, Serbia
  • Maja Josip Velhner Scientific Veterinary Institute „Novi Sad“, Novi Sad, Serbia
  • Neđeljko Ratko Karabasil Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

Abstract

Introduction: The aim of this study was to determine the prevalence of Salmonella along the slaughter line and to identify possible critical control points in one slaughterhouse facility located in the city of Belgrade.


Methodology: In total, 700 samples were tested: two swabs from both sides of carcass were taken from each of 100 pigs. In this way, 200 pig skin swab samples were taken after stunning, 200 after processing and 200 after chilling. Additional 100 samples of ileal contents were also taken from the same pigs to obtain a collection of 270 isolates. All samples were analyzed using standard culture methods and serotyping.  PFGE was performed for 27 isolates. Determination of antimicrobial resistance was performed by E-test.


Results: In total, 47 (23.5%) swab samples were positive for the presence of Salmonella after stunning. After processing, Salmonella was isolated in two swab samples (1%), whereas all samples which were collected after chilling were negative for Salmonella. The sampling of ileal contents was positive for five Salmonella isolates (5%). The most frequently isolated serotypes were S. Derby (90.74%), S. Infantis (5.56%) and S. Typhimurium (3.7%). All tested isolates were resistant to tetracycline. Resistance was recorded to nalidixic acid (23.3%), ciprofloxacin (20%), ampicillin (10%) and chloramphenicol (14.4%), as well. The PFGE results indicated that isolates had a high genetic similarity.


Conclusions: The investigation has confirmed that bacteriological examinations of carcass swabs, as well as ileal content, could be used to assess the carriage of salmonellae in pigs at the time of slaughter.

Author Biographies

Jasna Mirko Kureljusic, Institute of Veterinary Medicine of Serbia, Belgrade, Serbia

Department for food microbiology

Marko Predrag Dmitrić, Veterinary Specialized Institute “Kraljevo”, Kraljevo, Serbia

Department for Food Microbiology

Dejan Svetislav Vidanović, Veterinary Specialized Institute “Kraljevo“, Kraljevo, Serbia

Department for Food Microbiology

Vlado Božidar Teodorović, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia

Department for Food Hygiene and Technology

Branislav Ilija Kureljušić, Institute of Veterinary Medicine of Serbia, Belgrade, Serbia

Pathology Department

Maja Josip Velhner, Scientific Veterinary Institute „Novi Sad“, Novi Sad, Serbia

Microbiology Department

Neđeljko Ratko Karabasil, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
Department for Food Hygiene and Technology
Published
2017-09-05
How to Cite
Kureljusic J, Dmitrić M, Vidanović D, Teodorović V, Kureljušić B, Velhner M, Karabasil N (2017) Prevalence of Salmonella enterica in slaughtered pigs in Serbia: Serotyping, PFGE-genotyping and antimicrobial resistance. The Journal Of Infection In Developing Countries 11 (08): 640-645. https://doi.org/10.3855/jidc.9311
Section
Original Articles

Keywords

pig; slaughter; Salmonella; antimicrobial resistance; PFGE