Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

  • Casmir Ifeanyichukwu Cajetan Ifeanyi Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria
  • Nkiruka Florence Ikeneche College of Health Science, University of Abuja, Abuja, Nigeria
  • Bassey Enya Bassey World Health Organization, Nigeria Country Office, UN House, Plot 617/618 Diplomatic Drive, Central Business District, Garki, Abuja, Nigeria
  • Stefano Morabito Istituto Superiore di Sanita, Rome, Italy
  • Caterina Graziani Istituto Superiore di Sanita, Rome, Italy
  • Alfredo Caprioli Istituto Superiore di Sanita, Rome, Italy

Abstract




Introduction: Enteropathogenic Escherichia coli (EPEC) causes infectious diarrhea among children in developing countries. However, in Nigeria, due to limited laboratory resources, the genetic diversity of its virulence factors, which includes its intimin subtypes remain undefined. This study aims to evaluate the serotypes, antibiotic resistance and the intimin subtypes genes associated with EPEC strains isolated from children with acute diarrhea in Abuja, Nigeria.


Methodology: EPEC isolates from diarrheic children aged 0-60 months in Abuja, Nigeria were analyzed. PCR for EPEC virulence gene, Hep-2 cell Adherence and serotyping were performed. EPEC strains were further subtyped by PCR reactions for the identification of intimin subtype genes: α (alpha), β (beta), γ1 (gamma-1), and έ (epsilon). Antibiotic resistance and ESBL production was determined by CLSI guidelines.


Results: Overall, 18 (4.5%) out of 400 children with acute diarrhea had EPEC infection. Typical EPEC strains were detected in 14 (3.5%) whereas 4 (1.1%) were atypical EPEC. Fifteen (83.3%) of the EPEC isolated belonged to β intimin subtype gene, while the remaining 3 EPEC isolates possessed the intimin έ subtype. No α and γ intimin subtypes were detected. Traditional EPEC serotypes O114:H14 were detected only in tEPEC strains. Marked resistant to β-lactam agents were observed but no ESBL-producing tEPEC or aEPEC was detected.


Conclusions: This is the first report of intimin subtype genes in Abuja, Nigeria. These data will be useful in facilitating the characterization of intimin variants of EPEC and some Shiga toxin-producing E. coli (STEC) in humans and other animal species.


 



Introduction: Enteropathogenic Escherichia coli (EPEC) causes infectious diarrhea among children in developing countries. However, in Nigeria, due to limited laboratory resources, the genetic diversity of its virulence factors, which includes its intimin subtypes remain undefined. This study aims to evaluate the serotypes, antibiotic resistance and the intimin subtypes genes associated with EPEC strains isolated from children with acute diarrhea in Abuja, Nigeria.


 


Methodology: EPEC isolates from diarrheic children aged 0-60 months in Abuja, Nigeria were analyzed. PCR for EPEC virulence gene, Hep-2 cell Adherence and serotyping were performed. EPEC strains were further subtyped by PCR reactions for the identification of intimin subtype genes: α (alpha), β (beta), γ1 (gamma-1), and έ (epsilon). Antibiotic resistance and ESBL production was determined by CLSI guidelines.


 


Results: Overall, 18 (4.5%) out of 400 children with acute diarrhea had EPEC infection. Typical EPEC strains were detected in 14 (3.5%) whereas 4 (1.1%) were atypical EPEC. Fifteen (83.3%) of the EPEC isolated belonged to β intimin subtype gene, while the remaining 3 EPEC isolates possessed the intimin έ subtype. No α and γ intimin subtypes were detected. Traditional EPEC serotypes O114:H14 were detected only in tEPEC strains. Marked resistant to β-lactam agents were observed but no ESBL-producing tEPEC or aEPEC was detected.


 


Conclusions: This is the first report of intimin subtype genes in Abuja, Nigeria. These data will be useful in facilitating the characterization of intimin variants of EPEC and some Shiga toxin-producing E. coli (STEC) in humans and other animal species.

Author Biographies

Casmir Ifeanyichukwu Cajetan Ifeanyi, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria

Research Scientist Department of Microbiology Faculty of Veterinary Medicine University of Abuja, Nigeria

Nkiruka Florence Ikeneche, College of Health Science, University of Abuja, Abuja, Nigeria

Department of Medical Microbiology,
College of Health Science

Stefano Morabito, Istituto Superiore di Sanita, Rome, Italy

EU Reference Laboratory for E. coli, Department of Veterinary Public Health and Food Safety,

Caterina Graziani, Istituto Superiore di Sanita, Rome, Italy

EU Reference Laboratory for E. coli, Department of Veterinary Public Health and Food Safety,

Alfredo Caprioli, Istituto Superiore di Sanita, Rome, Italy

EU Reference Laboratory for E. coli, Department of Veterinary Public Health and Food Safety

Published
2017-07-31
How to Cite
Ifeanyi C, Ikeneche N, Bassey B, Morabito S, Graziani C, Caprioli A (2017) Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria. The Journal Of Infection In Developing Countries 11 (07): 527-535. https://doi.org/10.3855/jidc.9338
Section
Original Articles

Keywords

Gastroenteritis, Enteropathogenic, Intimin gene, Bundle-forming pilus