Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran

  • Mozhgan Esmaeili Benvidi Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Hamidreza Houri Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Zohreh Ghalavand Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Bahram Nikmanesh Tehran University of Medical Sciences, Tehran, Iran
  • Hadi Azimi Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Roghayeh Samadi Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Narges Nodeh Farahani Iran University of Medical Sciences, Tehran, Iran
  • Gita Eslami Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Introduction: Staphylococcus aureus is known to be a major cause of skin and soft tissue infections, pneumonia and invasive diseases. In this study, attempts were made to examine the prevalence of tsst-1, eta, etb, and luk-PV genes among methicillin-resistant S. aureus (MRSA) isolated from children in Tehran.


Methodology: In the present cross-sectional study, a total of 100 MRSA were isolated from children who were referred to a pediatric hospital during 11-month period of September 2014 to August 2015. Isolates were identified using biochemical tests and then, using PCR, the isolates were tested for the presence of mecA, tsst-1, eta, etb, and luk-PV genes. Susceptibility of isolates to cefoxitin, penicillin, erythromycin, clindamycin, gentamicin, rifampin, minocycline, co-trimoxazole, linezolid, and vancomycin were evaluated using standard methods.


Results: It was found that the MRSA isolates had the greatest resistance to clindamycin (72%) and erythromycin (59%), while the lowest rates of resistance were observed to be related to minocycline (6%) and rifampin (12%). All of isolates were sensitive to vancomycin and linezolid. The mecA gene was detected in all the isolates. Moreover, luk-PV and tsst-1 were detected in 18% and 17% of the isolates, respectively. None of the isolates harbored eta and etb genes.


Conclusions: Our data provide specifications about the toxin production status of S. aureus isolates from pediatric children. The current study showed increased resistance to different antibiotics in S. aureus isolates. Therefore, to prevent multi-resistance to other antibiotic classes, it is essential to withhold prescriptions and stop unessential use of available antibiotics.

Author Biographies

Mozhgan Esmaeili Benvidi, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Department of Microbiology, School of Medicine

Hamidreza Houri, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Department of Microbiology, School of Medicine

Zohreh Ghalavand, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Department of Microbiology, School of Medicine

Bahram Nikmanesh, Tehran University of Medical Sciences, Tehran, Iran

Department of Medical Laboratory Sciences, School of Allied Medical Sciences

Hadi Azimi, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Department of English Language Teaching, School of Medicine

Roghayeh Samadi, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Department of Microbiology, School of Medicine

Narges Nodeh Farahani, Iran University of Medical Sciences, Tehran, Iran

Department of Microbiology, School of Medicine

Gita Eslami, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Department of Microbiology, School of Medicine

Published
2017-11-06
How to Cite
Esmaeili Benvidi M, Houri H, Ghalavand Z, Nikmanesh B, Azimi H, Samadi R, Nodeh Farahani N, Eslami G (2017) Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran. The Journal Of Infection In Developing Countries 11 (10): 759-765. https://doi.org/10.3855/jidc.9360
Section
Original Articles

Keywords

Methicillin-Resistant Staphylococcus aureus; Toxic shock syndrome toxin-1; Panton-valentine leukocidin; Staphylococcal exfoliative toxin; Pediatrics.