First detection of Zika virus infection in a Croatian traveler returning from Brazil, 2016

Imported Zika virus infection in Croatia

  • Tatjana Vilibic-Cavlek Croatian National Institute of Public Health, Zagreb, Croatia
  • Ljiljana Betica-Radic General Hospital Dubrovnik, Dubrovnik, Croatia
  • Giulietta Venturi Istituto Superiore di Sanità, Roma, Italia
  • Claudia Fortuna Istituto Superiore di Sanità, Roma, Italia
  • Stjepan Djuricic General Hospital Dubrovnik, Dubrovnik, Croatia
  • Antonella Salvia-Milos General Hospital Dubrovnik, Dubrovnik, Croatia
  • Irena Tabain Croatian National Institute of Public Health, Zagreb, Croatia
  • Ljubo Barbic Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
  • Vladimir Stevanovic Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
  • Eddy Listes Croatian Veterinary Institute, Regional Institute Split, Split, Croatia
  • Giovanni Savini Istituto Zooprofillatico Sperimentale "G. Caporale", Teramo, Italia

Abstract

In the last few years, several imported cases of Zika virus (ZIKV) infection were reported in European countries. We report the first imported ZIKV infection case in a Croatian traveler returning from Brazil. The patient presented with a low-grade fever, pruritic rash, general weakness, myalgia, arthralgia and edema of the legs and recovered completely within a week. ZIKV infection was confirmed by detection of IgM/IgG antibodies using enzyme-linked immunosorbent assay (ELISA) and confirmed by plaque-reduction neutralization test (PRNT). ZIKV IgM antibodies cross-reacted with dengue virus (DENV), West Nile virus (WNV) and tick-borne encephalitis virus (TBEV) in ELISA. In indirect immunofluorescence assay (IFA), IgM cross-reactivity was found only with DENV-3.  ZIKV IgG antibodies cross-reacted with DENV in both ELISA and IFA. PRNT for DENV was negative. Control serology performed on days 64 and 98 after disease onset showed a decline in cross-reactive heterologous DENV IgG antibodies compared to persistently high titer of homologous ZIKV IgG antibodies.

Author Biographies

Tatjana Vilibic-Cavlek, Croatian National Institute of Public Health, Zagreb, Croatia

Department of Virology

School of Medicine University of Zagreb

Ljiljana Betica-Radic, General Hospital Dubrovnik, Dubrovnik, Croatia

Department of Infectious Diseases

Stjepan Djuricic, General Hospital Dubrovnik, Dubrovnik, Croatia

Department of Infectious Diseases

Antonella Salvia-Milos, General Hospital Dubrovnik, Dubrovnik, Croatia

Department of Infectious Diseases

Irena Tabain, Croatian National Institute of Public Health, Zagreb, Croatia

Department of Virology

Ljubo Barbic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine

Vladimir Stevanovic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine

Eddy Listes, Croatian Veterinary Institute, Regional Institute Split, Split, Croatia

Croatian Veterinary Institute

Published
2017-09-05
How to Cite
Vilibic-Cavlek T, Betica-Radic L, Venturi G, Fortuna C, Djuricic S, Salvia-Milos A, Tabain I, Barbic L, Stevanovic V, Listes E, Savini G (2017) First detection of Zika virus infection in a Croatian traveler returning from Brazil, 2016. The Journal Of Infection In Developing Countries 11 (08): 662-667. https://doi.org/10.3855/jidc.9410
Section
Case Reports

Keywords

Zika virus; imported; Croatia