Virulence genes and resistance to antibiotics of beta-hemolytic streptococci isolated from children in Chiapas, Mexico

  • Javier Gutiérrez-Jiménez Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México
  • Mónica Ivonne Mendoza-Orozco Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México
  • Alejandra Vicente-Serrano Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México
  • Lorena Mercedes Luna-Cazáres Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México
  • José Manuel Feliciano-Guzmán Hospital de Especialidades Pediátricas, Tuxtla Gutiérrez, Chiapas, México
  • Jose Antonio Girón-Hernández Hospital de Especialidades Pediátricas, Tuxtla Gutiérrez, Chiapas, México
  • Jorge Eugenio Vidal Emory University, Atlanta GA, United States
Keywords: Streptococcus pyogenes, virulence factors, antimicrobial susceptibility, Chiapas

Abstract

Introduction: Among beta-hemolytic streptococci, Streptococcus pyogenes causes a wide variety of human disease including pharyngitis, necrotizing fasciitis and streptococcal toxic syndrome. Group A Streptococcus (GAS) uses a variety of virulence traits to colonize and then cause damage to the host; others species of beta-hemolytic streptococci are considered as emerging pathogens for humans. Despite its recognized virulence, only few studies have investigated virulence factors of GAS strains isolated in Mexico.

Methodology: We conducted an epidemiological study to investigate the prevalence of GAS strains in child illnesses in Chiapas Mexico. Virulence genes encoding proteases, DNases, superantigens, as well as susceptibility to antibiotics were investigated.

Results: During 2010, 2013 and 2014, beta-hemolytic streptococci (N=12) were isolated from cases of bacterial infections including pharyngitis and bacteremia, with a prevalence of 0.42, 0.04 and 0.20%, respectively. S. pyogenes was the most frequent species (33%) followed by S. agalactiae and S. dysgalactiae subsp. equisimilis (25%, each). Most GAS strains encoded genes for proteases: scpA, speB, spyCEP and mac (75%), followed by sdaD and sdaB (DNases) (50%), speA and speG (superantigens; 50 and 25%, respectively). The scpA gene was amplified in all S. agalactiae strains and in ~35% of SDSE strains. Strains were all susceptible to beta-lactams, cephalosporins and quinolones.

Conclusions: The present study provides evidence on the epidemiology of beta-hemolytic streptococci infecting children at the southeast Mexico, their virulence traits and sensitivity to first-line antibiotics.

Author Biographies

Javier Gutiérrez-Jiménez, Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México

Professor in Microbiology

Laboratorio de Biología Molecular y Genética, Instituto de Ciencias Biológicas

Mónica Ivonne Mendoza-Orozco, Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México

Instituto de Ciencias Biológicas, Biologist.

Alejandra Vicente-Serrano, Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México

Instituto de Ciencias Biológicas

Lorena Mercedes Luna-Cazáres, Universidad de Ciencias y Artes de Chiapas (UNICACH), Chiapas, México

Instituto de Ciencias Biológicas

José Manuel Feliciano-Guzmán, Hospital de Especialidades Pediátricas, Tuxtla Gutiérrez, Chiapas, México

Laboratorio de Microbiología y Patología

Jorge Eugenio Vidal, Emory University, Atlanta GA, United States

Hubert Department of Global Health, Rollins School of Public Health

Published
2018-02-28
How to Cite
1.
Gutiérrez-Jiménez J, Mendoza-Orozco M, Vicente-Serrano A, Luna-Cazáres L, Feliciano-Guzmán J, Girón-Hernández J, Vidal J (2018) Virulence genes and resistance to antibiotics of beta-hemolytic streptococci isolated from children in Chiapas, Mexico. J Infect Dev Ctries 12:80-88. doi: https://doi.org/10.3855/jidc.9679
Section
Original Articles