Dissemination of ST101 blaOXA-48 producing Klebsiella pneumoniae at tertiary care setting

  • Hadir ElMahallawy National Cancer Institute, Cairo University, Cairo, Egypt
  • Mai Mahmoud Zafer Ahram Canadian University, Giza, Egypt
  • Mohamed Al-Agamy King Saud University, Riyadh, Saudi Arabia
  • Magdy Aly Amin Cairo University, Cairo, Egypt
  • Mai Muhammed Mersal MTI university, Cairo, Egypt
  • Rayan Yousef Booq King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia
  • Essam Alyamani
  • Samah Radwan
Keywords: K. pneumoniae, blaOXA48, blaNDM-1, ST101, ST383, Egypt

Abstract

Introduction: The worldwide dissemination of the acquired carbapenemases in Gram-negative bacteria is a strongly expressed demand for the emergence of post antibiotic era. The aim of this study was to test the production of carbapenemase by Klebsiella pneumoniae strains isolated from hospitalized cancer patients and to investigate the genetic relationship of carbapenemase producing carbapenem resistant K. pneumoniae using multilocus sequence typing (MLST).

Methodology: Antibiotic susceptibility testing and phenotypic testing for extended spectrum b-lactamases (ESBL) and carbapenemases production were performed. PCR amplification of ESBL and carbapenemase genes was performed. MLST was done to detect the genetic relatedness of the isolates.

Results: Our data showed all strains were sensitive to colistin. Carba NP test was positive in thirty-one carbapenem resistant K. pneumoniae isolates and 26 out of 34 K. pneumoniae isolates were metallo-beta-lactamases (MBL) positive. All carbapenemase-positive isolates were ESBL CTX-M-1-like positive. blaOXA-48 gene was detected in 25 isolates (80.65%) and 21 isolates (67.75%) produced blaNDM-1 like enzyme. VIM and KPC genes were not identified in this study. Association of blaOXA-48 like and blaNDM-1 like was found in 15 (48.39%) isolates, while the coproduction of OXA-48-like and IMP-1 was revealed in only one K. pneumoniae isolate. MLST revealed ten distinct sequence types (STs).

Conclusion: Here we have documented the coexistence of NDM-type and OXA-48-like, and the coproduction of OXA-48-like and IMP in carbapenem resistant K. pneumoniae in patients with cancer. The dominant clone of the OXA-48-like-producing K. pneumoniae isolates from Egypt was ST101 epidemic clone belonging to clonal complex 101, an association that has been reported worldwide. The second most frequent ST was ST383.ST11 was assigned to OXA-48-producing K. pneumoniae.

Author Biographies

Hadir ElMahallawy, National Cancer Institute, Cairo University, Cairo, Egypt

Department of Clinical Pathology

Mai Mahmoud Zafer, Ahram Canadian University, Giza, Egypt

Department of Microbiology and Immunology, Faculty of Pharmacy

Mohamed Al-Agamy, King Saud University, Riyadh, Saudi Arabia

Department of Pharmaceutics, College of Pharmacy

Magdy Aly Amin, Cairo University, Cairo, Egypt

Department of Microbiology and Immunology, Faculty of Pharmacy

Mai Muhammed Mersal, MTI university, Cairo, Egypt

Department of Microbiology and Immunology, Faculty of pharmacy

Rayan Yousef Booq, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia

National Center for Biotechnology

Published
2018-06-30
How to Cite
1.
ElMahallawy H, Zafer M, Al-Agamy M, Amin M, Mersal M, Booq R, Alyamani E, Radwan S (2018) Dissemination of ST101 blaOXA-48 producing Klebsiella pneumoniae at tertiary care setting. J Infect Dev Ctries 12:422-428. doi: https://doi.org/10.3855/jidc.9789
Section
Original Articles