High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria

  • Tunmise O. Ayandiran University of Ibadan, Ibadan, Oyo State, Nigeria
  • Linda Falgenhauer Justus Liebig University and German Center for Infection Research, Partner site Giessen-Marburg-Langen, Giessen, Germany
  • Judith Schmiedel Justus Liebig University and German Center for Infection Research, Partner site Giessen-Marburg-Langen, Giessen, Germany
  • Trinad Chakraborty Justus Liebig University and German Center for Infection Research, Partner site Giessen-Marburg-Langen, Giessen, Germany
  • Funmilola Abidemi Ayeni University of Ibadan, Ibadan, Oyo State, Nigeria
Keywords: resistance, poultry, Tetracycline, Ciprofloxacin

Abstract

Introduction: Resistance to ciprofloxacin and tetracycline is increasing in the food chain especially in E. coli strains and more worrisome will be occurrence of extended-spectrum beta-lactamase (ESBL) producers among ciprofloxacin- and tetracycline-resistant isolates. This study was undertaken to investigate the occurrence and mechanism of ciprofloxacin-, tetracycline- and ESBL-resistant bacteria in poultry in Ibadan, Nigeria.

Methodology: Bacteria were isolated from poultry feces in two farms in Ibadan and identified by MALDI-TOF. Antibiotic susceptibility patterns of the isolates were determined by disc diffusion and Minimum Inhibitory Concentration (MIC) using Vitek-2 apparatus. Four tetracycline genes and six plasmids mediated quinolone resistance genes (PMQR) were investigated by PCR. Whole genome sequencing was done for strains that were ESBL producers.

Results: Bacterial strains (≥ 105 cfu/mL) were counted on ciprofloxacin and tetracycline supplemented plates. 106 bacteria from 14 different species were identified with high resistance to quinolones, tetracycline and trimethoprim. 49% of the strains were E. coli with 90% resistance for nalidixic acid, moxifloxacin (94%), ciprofloxacin (88%) levofloxacin (78%) and tetracycline (77%). The genes tetA, tetB, qnrB, qnrS and qepA were detected with 37%, 4%, 35%, 4% and 2% prevalence in E. coli respectively. Three ESBL-producing E. coli of the sequence type ST-6359 were found and harboured blaCTX-M-15 located in the chromosome, at the same insertion site. All the ESBL producers harboured mutations in gyrA (S83L/D87N/D678E) and parC (S80I).

Conclusion: The observed high quinolones and tetracycline resistance with ESBL producers in this study calls for caution in the use of these antibiotics in poultry feeds.

Author Biographies

Tunmise O. Ayandiran, University of Ibadan, Ibadan, Oyo State, Nigeria

Department of Pharmaceutical Microbiology, Faculty of Pharmacy

Linda Falgenhauer, Justus Liebig University and German Center for Infection Research, Partner site Giessen-Marburg-Langen, Giessen, Germany

Institute of Medical Microbiology

Judith Schmiedel, Justus Liebig University and German Center for Infection Research, Partner site Giessen-Marburg-Langen, Giessen, Germany

Institute of Medical Microbiology

Trinad Chakraborty, Justus Liebig University and German Center for Infection Research, Partner site Giessen-Marburg-Langen, Giessen, Germany

Institute of Medical Microbiology

Funmilola Abidemi Ayeni, University of Ibadan, Ibadan, Oyo State, Nigeria

Department of Pharmaceutical Microbiology, Faculty of Pharmacy

Faculty of Pharmacy

Lecturer I

Published
2018-06-30
How to Cite
1.
Ayandiran T, Falgenhauer L, Schmiedel J, Chakraborty T, Ayeni F (2018) High resistance to tetracycline and ciprofloxacin in bacteria isolated from poultry farms in Ibadan, Nigeria. J Infect Dev Ctries 12:462-470. doi: https://doi.org/10.3855/jidc.9862
Section
Original Articles