Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China

  • Chunyan Jiang Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Xiaoju Zhang Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Jianfeng Han Chinese Academy of Agricultural Sciences, Beijing, China
  • Haijian He Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Chaoying Zhang Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Hongbing Zhang Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Jingjing Jin Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Liang Wang Shanxi Yuncheng Agricultural Vocational And Technical College, Yuncheng, Shanxi, China
  • Bingqian Ge Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Yanli Wang Jinhua Polytechnic, Jinhua, Zhejiang, China
  • Yongjie Liu Nanjing Agricultural University, Nanjing, Jiangsu, China
Keywords: Swine diarrhea, porcine epidemic diarrhea virus, S1 gene, genetic evolution analysis

Abstract

Introduction: Porcine epidemic diarrhea virus (PEDV) is one of the most common viral pathogens causing swine diarrhea.

Methodology: We performed a genetic evolution analysis of the S1 gene of endemic PEDV strains in Eastern China. The S1 genes of 37 PEDV-positive samples were amplified and sequenced, and compared to the standard CV777 strain, 120 nucleotides were found to have mutations.

Results: The nucleotide and deduced amino acid homologies between the sequences and those of the CV777 strain were 90%–91% and 88.2%–90%, respectively, and their homologies to the vaccine strain were 88.6%–89.7% and 86.2%–87.8%, respectively. Genetic evolution and variation analyses indicated that the 37 PEDV strains belonged to genogroup 2-1, while the CV777 strain, vaccine strain, and earlier Chinese strains all belonged to genogroup 1-1.

Conclusions: The newly emerged clinical PEDV strains indicate that the PEDV CV777 vaccine currently used in China may not fully protect pigs from infection with recent epidemic strains, and will require the development of new vaccine strains.

Author Biographies

Chunyan Jiang, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Xiaoju Zhang, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Jianfeng Han, Chinese Academy of Agricultural Sciences, Beijing, China

Feed Research Institute

Haijian He, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Chaoying Zhang, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Hongbing Zhang, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Jingjing Jin, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Liang Wang, Shanxi Yuncheng Agricultural Vocational And Technical College, Yuncheng, Shanxi, China

Department of Agriculture and Forestry Engineering

Bingqian Ge, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Yanli Wang, Jinhua Polytechnic, Jinhua, Zhejiang, China

Department of Animal Production, Faculty of Agriculture and Bioengineering

Yongjie Liu, Nanjing Agricultural University, Nanjing, Jiangsu, China

College of Veterinary Medicine

Published
2018-12-31
How to Cite
1.
Jiang C, Zhang X, Han J, He H, Zhang C, Zhang H, Jin J, Wang L, Ge B, Wang Y, Liu Y (2018) Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus isolates in Eastern China. J Infect Dev Ctries 12:1124-1133. doi: https://doi.org/10.3855/jidc.9898
Section
Original Articles